Composite

Part:BBa_K2092009:Design

Designed by: Hui Wen Chong   Group: iGEM16_Manchester   (2016-10-02)


PalcA(var) + RBS (B0034) +amilCP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 274
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

Aspergillus nidulans. The promoter was first characterized by ?, codon optimized for E.coli by Thermo Fischer Scientific and synthesized by IDT. The construct was assembled using the 3A BioBrick Assembly Method as recommended by iGEM.

References